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Accueil > Bibliographie > Evidence for an internal and functional circadian clock in rat pituitary (...)

Evidence for an internal and functional circadian clock (...)

Mol Cell Endocrinol. 2014 Feb ;382(2):888-98
Evidence for an internal and functional circadian clock in rat pituitary cells.
Becquet D, Boyer B, Rasolonjanahary R, Brue T, Guillen S, Moreno M, Franc JL, Francois-Bellan AM.

In primary cultures of rat pituitary cells and in a pituitary sommatolactotroph cell line (GH4C1), endogenous core-clock- as well as hormone-genes such as prolactin displayed a rhythmic expression pattern, fitted by a sinusoidal equation in which the period value was close to the circadian one. This is consistent with the presence of a functional circadian oscillator in pituitary cells whose importance was ascertained in GH4C1 cell lines stably expressing a dominant negative mutant of BMAL1. In these cells, both endogenous core-clock- and prolactin-genes no more displayed a circadian pattern. Some genes we recently identified as mouse pituitary BMAL1-regulated genes in a DNA-microarray study, lost their circadian pattern in these cells, suggesting that BMAL1 controlled these genes locally in the pituitary. The intra-pituitary circadian oscillator could then play a role in the physiology of the gland that would not be seen anymore as a structure only driven by hypothalamic rhythmic control.

PubMed

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